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        1. 首頁(yè) / 細(xì)胞轉(zhuǎn)染 / 產(chǎn)品應(yīng)用詳情

          PEI轉(zhuǎn)染試劑與脂質(zhì)體轉(zhuǎn)染試劑選擇指南

          產(chǎn)品概述

          PEI轉(zhuǎn)染試劑與脂質(zhì)體轉(zhuǎn)染試劑是目前使用最廣泛的化學(xué)類(lèi)細(xì)胞轉(zhuǎn)染試劑,在基因功能研究、基因表達(dá)調(diào)控、突變分析,以及基因治療、細(xì)胞治療、蛋白生產(chǎn)、疫苗生產(chǎn)等方面應(yīng)用廣泛。

          針對(duì)DNA轉(zhuǎn)染試劑與RNA轉(zhuǎn)染試劑,翌圣均擁有雄厚的研發(fā)與生產(chǎn)團(tuán)隊(duì),不斷優(yōu)化配方,改良生產(chǎn)工藝,推出了多款以陽(yáng)離子脂質(zhì)體與陽(yáng)離子聚合物為基礎(chǔ)的產(chǎn)品,為廣大科研院校與企業(yè)提供全方位的產(chǎn)品,產(chǎn)品線覆蓋轉(zhuǎn)染試劑涉及的各個(gè)領(lǐng)域。

           

          產(chǎn)品優(yōu)勢(shì)

           

          高效性:適合瞬時(shí)轉(zhuǎn)染或者穩(wěn)定轉(zhuǎn)染細(xì)胞系。

          低毒性:轉(zhuǎn)染的細(xì)胞仍保持很好的活性。

          適應(yīng)性廣:普通細(xì)胞、難轉(zhuǎn)染的原代細(xì)胞全面覆蓋。

          操作簡(jiǎn)便:適合血清存在的培養(yǎng)基,轉(zhuǎn)染前后無(wú)需更換培養(yǎng)基。

          性價(jià)比高:經(jīng)濟(jì)實(shí)用,轉(zhuǎn)染效率高,價(jià)格低。

           

          選擇指南

           

          轉(zhuǎn)染試劑的選擇,需要根據(jù)實(shí)驗(yàn)?zāi)康呐c實(shí)驗(yàn)內(nèi)容,如轉(zhuǎn)染的物質(zhì)、具體的細(xì)胞、操作的便捷性等因素綜合考量,小編進(jìn)行了以下的區(qū)分,供大家參考。

          1、DNA/質(zhì)粒轉(zhuǎn)染試劑

          A10 kb以下DNA/質(zhì)粒

          產(chǎn)品名稱(chēng)

          核酸類(lèi)型

          細(xì)胞類(lèi)型

          優(yōu)點(diǎn)

          貨號(hào)

          規(guī)格

          Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑

          DNA(小于10 kb

          貼壁細(xì)胞

          1.轉(zhuǎn)染效率高
          2.用量少

          40802ES02/03/08

          0.5 mL/1 mL/5×1 mL

          磷酸鈣法細(xì)胞轉(zhuǎn)染試劑

          DNA(小于10 kb

          貼壁細(xì)胞

          1.毒性低
          2.操作簡(jiǎn)單
          3.用量少

          40803ES70

          200 T

          懸浮細(xì)胞專(zhuān)用脂質(zhì)體核酸轉(zhuǎn)染試劑

          DNA(小于10 kb

          懸浮細(xì)胞

          1.專(zhuān)門(mén)轉(zhuǎn)染懸浮細(xì)胞
          2.毒性低
          3.轉(zhuǎn)染效率高

          40805ES02/03/08

          0.5 mL/1 mL/5×1 mL

          Hieff Trans®通用型轉(zhuǎn)染試劑

          DNA(小于10 kb

          貼壁和懸浮細(xì)胞

          1.毒性低
          2.用量少

          40808ES02/03/08

          0.5 mL/1 mL/5×1 mL

           

          Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑

           

          Hieff Trans®脂質(zhì)體核酸轉(zhuǎn)染試劑是一種多用途的脂質(zhì)體轉(zhuǎn)染試劑,適用于DNA、RNA和寡核苷酸的轉(zhuǎn)染,對(duì)大多數(shù)真核細(xì)胞具有很高的轉(zhuǎn)染效率。其獨(dú)特的配方使其可直接加入培養(yǎng)基中,血清的存在不會(huì)影響轉(zhuǎn)染效率,這樣可以減少去除血清對(duì)細(xì)胞的損傷。轉(zhuǎn)染后不需要除去核酸-脂質(zhì)體復(fù)合物或更換新鮮培養(yǎng)基,也可在46小時(shí)后除去。

           

          1. Hieff Trans® 轉(zhuǎn)染試劑操作流程示意圖

          本產(chǎn)品以無(wú)菌的液體形式提供,通常情況下對(duì)于24孔板轉(zhuǎn)染,每次用1.5 μL左右,則1 mL約可做660次轉(zhuǎn)染;對(duì)于6孔板,每次用4 μL左右,則1 mL 約可做250次轉(zhuǎn)染;

           

          B、10 kb以上DNA/質(zhì)粒

          產(chǎn)品名稱(chēng)

          核酸類(lèi)型

          細(xì)胞類(lèi)型

          優(yōu)點(diǎn)

          貨號(hào)

          規(guī)格

          線性PEI轉(zhuǎn)染試劑MW25000

          DNA(大于10 kb的也可以轉(zhuǎn)染)

          貼壁細(xì)胞(較好)
          懸浮細(xì)胞(一般)

          1.轉(zhuǎn)染效率高
          2.用量少

          40815ES03/08

          1 g/5×1 g

          線性PEI轉(zhuǎn)染試劑MW40000(速溶型)

          DNA(大于10 kb的也可以轉(zhuǎn)染)

          貼壁細(xì)胞(較好)
          懸浮細(xì)胞(一般)

          1.轉(zhuǎn)染效率高
          2..用量少

          40816ES02/03

          100 mg/1 g

           

          Polyethylenimine Linear (PEI) MW40000rapid lysis) 線性PEI轉(zhuǎn)染試劑(速溶型)MW40000

          PEI 40000是一種分子量為40000的高電荷陽(yáng)離子聚合物,非常容易結(jié)合帶負(fù)電荷的核酸分子,形成復(fù)合物,并使該復(fù)合物進(jìn)入細(xì)胞中。PEI 40000是一種瞬時(shí)轉(zhuǎn)染試劑,細(xì)胞毒性低,轉(zhuǎn)染效率高,在HEK293CHO等細(xì)胞中基因表達(dá)效率較高。目前已經(jīng)驗(yàn)證線性PEI轉(zhuǎn)染試劑廣泛適用于多種細(xì)胞系包括HEK-293HEK293T、CHO-K1COS-1、COS-7NIH/3T3 、Sf9HepG2Hela細(xì)胞等。轉(zhuǎn)染效率高達(dá)80%~90%

           

          2、RNA轉(zhuǎn)染試劑

          產(chǎn)品名稱(chēng)

          核酸類(lèi)型

          細(xì)胞類(lèi)型

          優(yōu)點(diǎn)

          貨號(hào)

          規(guī)格

          Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑

          siRNA

          貼壁細(xì)胞

          1.轉(zhuǎn)染效率高
          2.用量少

          40802ES02/03/08

          0.5 mL/1 mL/5×1 mL

          Hieff Trans® siRNA/miRNA體外轉(zhuǎn)染試劑

          siRNA、miRNApre-miRNA, mimic miRNA, antimiRNA

          貼壁和懸浮細(xì)胞

          1.極好的基因沉默效率
          2.siRNA濃度低
          3.細(xì)胞毒性低

          40806ES02/03

          0.5 mL/1 mL

          Hieff Trans® mRNA轉(zhuǎn)染試劑

          mRNA

          貼壁和懸浮細(xì)胞

          1.轉(zhuǎn)染效率高
          2.用量少

          40809ES01/03

          0.1 mL/1 mL

           

          Hieff Trans® siRNA/miRNA體外轉(zhuǎn)染試劑

          Hieff Trans® siRNA/miRNA體外轉(zhuǎn)染試劑是一種非脂質(zhì)體PEI陽(yáng)離子、兩性分子轉(zhuǎn)染試劑,為siRNA轉(zhuǎn)染到哺乳動(dòng)物細(xì)胞中而開(kāi)發(fā)。適用于siRNAmiRNA的轉(zhuǎn)染。

          該產(chǎn)品可在廣泛的細(xì)胞系中,實(shí)現(xiàn)1 nMsiRNA超過(guò)90%的表達(dá)效率,避免了脫靶效應(yīng)。適用于多種細(xì)胞轉(zhuǎn)染,包括Hela、MCF-7、HepG2、CHO等貼壁細(xì)胞;以及難以轉(zhuǎn)染的懸浮細(xì)胞系,如K562THP-1細(xì)胞,可達(dá)到80%的沉默效率;同時(shí)還包括一些原代細(xì)胞,原代人成纖維細(xì)胞和原代人肝細(xì)胞等,可達(dá)到80%的沉默效率。

           

          3、DNA/RNA共轉(zhuǎn)染試劑

          產(chǎn)品名稱(chēng)

          核酸類(lèi)型

          細(xì)胞類(lèi)型

          優(yōu)點(diǎn)

          貨號(hào)

          規(guī)格

           Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑

          DNA(小于10 kb)、siRNA

          貼壁細(xì)胞

          1.轉(zhuǎn)染效率高
          2.用量少

          40802ES02/03/08

          0.5 mL/1 mL/5×1 mL

          Hieff Trans®通用型轉(zhuǎn)染試劑

          DNA(小于10 kb/siRNA

          貼壁和懸浮細(xì)胞

          1.毒性低
          2.用量少

          40808ES02/03/08

          0.5 mL/1 mL/5×1 mL

           

          4、工業(yè)生產(chǎn)轉(zhuǎn)染試劑系列產(chǎn)品

          產(chǎn)品名稱(chēng)

          使用場(chǎng)景(包括但不限于)

          細(xì)胞類(lèi)型

          優(yōu)點(diǎn)

          貨號(hào)

          規(guī)格

          線性PEI轉(zhuǎn)染試劑MW40000(速溶型)

          病毒載體、蛋白生產(chǎn)

          CHO系列、HEK-293系列

          1.轉(zhuǎn)染效率高
          2.用量少

          40816ES02/03

          100 mg/1 g

          Hieff Trans® PEI轉(zhuǎn)染試劑

          病毒載體生產(chǎn)(AAV/LV

          HEK-293系列

          1.轉(zhuǎn)染效率高
          2.用量少

          3.GMP級(jí)別

          40820ES04/10/60

          1.5 mL/10 mL/100 mL

           

           

          已驗(yàn)證細(xì)胞系列表

          產(chǎn)品名稱(chēng)

          Hieff Trans® Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑/40802ES

          已驗(yàn)證部分細(xì)胞系

          293T

          Calu 1

          HEK293

          HO1980

          N2A

          THP-1

          293T(懸浮)

          Chok1

          HEK293T

          HUVEC

          NCI-H1975

          TS cell

          293FT

          COS-7

          Hela

          MCF10A

          NIH-3T3

          U-87

          3T3

          DF-1

          Hep2C

          MCF-7

          Neuro-2a

          Vero

          A549

          H1299

          Hep3B

          MDA-MB-231

          PC-12

          WEHI

          BV-2

          H520

          Hepa1-6

          MDA-MB-231-LM2-4175 

          Raw264.7

          WRL-68

          B50

          HaCaT

          HepG2

          MDCK

          SGC-7901

          多發(fā)性骨髓瘤細(xì)胞

          C2C12

          HCT116

          HK2

          MEF

          T47D

           

          產(chǎn)品名稱(chēng)

          Hieff Trans® siRNA/miRNA體外轉(zhuǎn)染試劑/40806ES

          已驗(yàn)證部分細(xì)胞系

          A549

          HepG2

          MCF-7

          THP-1

           

           

          HEK293T

          CaSki

          RAW264.7

          原代人成纖維細(xì)胞

           

          Hela

          K562

          SiHA

          原代人干細(xì)胞

           

           

          產(chǎn)品名稱(chēng)

          線性PEI轉(zhuǎn)染試劑MW40000(速溶型)/40816ES

          已驗(yàn)證部分細(xì)胞系

          COS-1

          CHO-K1

          HEK-293

          HepG2

          Sf9

           

          COS-7

          Hela

          HEK-293T

          NIH-3T3

           

           

           

          拓展信息

           

          1、脂質(zhì)體與PEI轉(zhuǎn)染試劑的差異,可查看1分鐘解鎖兩代轉(zhuǎn)染試劑的差異
          2、轉(zhuǎn)染試劑常見(jiàn)問(wèn)題,可查看轉(zhuǎn)染效率低?小翊手把手教你做轉(zhuǎn)染實(shí)驗(yàn)太實(shí)用了!轉(zhuǎn)染試劑常見(jiàn)問(wèn)題大全,你想要的答案這里都有

          3Hieff Trans™ Liposomal Transfection Reagent 脂質(zhì)體核酸轉(zhuǎn)染試劑針對(duì)不同細(xì)胞中DNA與試劑用量參考,可查看40802參考數(shù)據(jù)

          4、線性PEI轉(zhuǎn)染試劑MW40000(速溶型)進(jìn)一步介紹,可查看轉(zhuǎn)染新寵——PEI MAX (線性PEI MW 40000)更高效的轉(zhuǎn)染試劑

          5、大牛們的高分文章選讀,可查看IF55分!翌圣轉(zhuǎn)染試劑助力高分文章翌圣明星CP--轉(zhuǎn)染試劑與PCR產(chǎn)品又登《Cell》期刊超燃!翌圣轉(zhuǎn)染試劑助力這個(gè)團(tuán)隊(duì)連發(fā)3IF10+文章

           

          產(chǎn)品使用發(fā)表的部分文獻(xiàn)

          向下滑動(dòng)查看

           

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          [2] Luo J, Yang Q, et al. TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022 Mar 17.41.582)西湖大學(xué)陶亮團(tuán)隊(duì)

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          [4] Chen S, Cao X, et al. circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c-Myc Translation. Adv Sci (Weinh). 2022 Jan 24.(IF:16.808)中國(guó)科學(xué)院北京生命科學(xué)研究院趙方慶團(tuán)隊(duì)

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          [9] Xie F, Su P, et al. Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as COVID-19 Therapy. Adv Mater. 2021 Oct 19. (IF:30.849)蘇州大學(xué)生物醫(yī)學(xué)研究院周芳芳團(tuán)隊(duì)和浙江大學(xué)生命科學(xué)研究院張龍團(tuán)隊(duì)

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          [11] Fan Y, Wang J, et al. CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer. 2021 Feb 2.(IF:27.403)南京醫(yī)科大學(xué)附屬逸夫醫(yī)院蘇東明團(tuán)隊(duì)

          [12] Dai L, Dai Y, et al. Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin. Mol Cell. 2021 Jul 1.(IF:17.97)浙江大學(xué)生命科學(xué)研究院黃俊團(tuán)隊(duì)和中科院生物物理所周政團(tuán)隊(duì)

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