Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55.

 

產(chǎn)品性質(zhì)

別名	TSG101; Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101
SwissProt ID	Q99816
基因ID	7251
免疫原	A synthetic peptide of human TSG101
緩沖液	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
分子量	Calculated MW:44 kDa; Observed MW: 44 kDa
克隆類(lèi)型	Monoclonal Antibody
克隆號(hào)	4D9
宿主	Rabbit
物種反應(yīng)	Human, Mouse, Rat
推薦應(yīng)用	WB, IHC-F, IHC-P, ICC/IF
純化方式	Affinity Purified

 

應(yīng)用

稀釋比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/50-1/200

 

儲(chǔ)存條件

-25~-15℃保存

 

注意事項(xiàng)

1. 避免反復(fù)凍融。

2. 本產(chǎn)品僅作科研用途。

3. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并佩戴一次性手套操作。

 

產(chǎn)品數(shù)據(jù)

Western blot

Western blot analysis of TSG101 in K562, Rat Brain, C6, 3T3, HeLa lysates using TSG101 antibody.

 

Immunocytochemistry

Immunocytochemistry analysis of TSG101(green) in HT-1080 using TSG101 antibody, and DAPI(blue).

 

Immunocytochemistry

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using TSG101 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

 

Ver.CN20231102