Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

 

產(chǎn)品性質(zhì)

別名	FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A
SwissProt ID	P0CG47
基因ID	7314
免疫原	A synthetic peptide of human K-48 Linkage Specific Ubiquitin.
緩沖液	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.
分子量	Refer to figures.
克隆類型	Monoclonal Antibody
亞型	IgG
宿主	Rabbit
物種反應(yīng)	Human, Mouse, Rat
推薦應(yīng)用	WB, IHC-F, IHC-P, ICC/IF
純化方式	Affinity Purified

 

應(yīng)用

稀釋比 WB:1/500-1/1000; IHC:1/50-1/100; IF: 1/50-1/200

 

儲存條件

-25~-15℃保存

 

注意事項

1. 避免反復(fù)凍融。

2. 本產(chǎn)品僅作科研用途。

3. 為了您的安全和健康，請穿實驗服并佩戴一次性手套操作。

 

產(chǎn)品數(shù)據(jù)
Western blot

Western blot analysis of K48 Linkage Specific Ubiquitin in C6, C2C12 lysates using Ubiquitin K48 antibody.

 

Western blot analysis of K48 Linkage Specfic Ubiquitin in 293 lysates using K48 Linkage Specific Ubiquitin antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using K48 Linkage Specific Ubiquitin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

 

Ver.CN20231220