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rProtein A/G Agarose Resin 蛋白A/G瓊脂糖純化樹脂

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產(chǎn)品簡介

天然蛋白A（Protein A）是一種發(fā)現(xiàn)于金黃色葡萄球菌的細胞壁表面蛋白，天然蛋白G（Protein G）是一種分離自G型或C型鏈球菌屬的細胞表面蛋白，二者功能相似，主要通過與免疫球蛋白（Ig）的Fc區(qū)相互作用，可結(jié)合大多數(shù)哺乳動物的IgG。然而兩者結(jié)合特異性上有所不同（詳細見附錄1，蛋白A，G對不同物種Ig的結(jié)合能力總表）。蛋白A，蛋白G常共價偶聯(lián)在固體載體如瓊脂糖珠或丙烯酸樹脂小珠上，直接用以做免疫沉淀（IP）或者免疫共沉淀（Co-IP）來進行蛋白功能相關(guān)研究，或者直接裝在層析柱上來純化單克隆或者多克隆抗體。

本品使用的是基因改造后的蛋白A和蛋白G，不僅維持其本身的Ig親和特性，同時也去除了天然蛋白本身的非主要結(jié)合域以降低非特異性結(jié)合。本品同時將蛋白A和蛋白G共價偶聯(lián)到瓊脂糖凝膠微球表面，比單獨的蛋白A或者蛋白G都有更廣的結(jié)合范圍，適合于所有蛋白A瓊脂糖珠和蛋白G瓊脂糖珠單獨可以結(jié)合的Ig，實用性更高。

翌圣為您提供蛋白純化實驗整體解決方案，相關(guān)產(chǎn)品選購請參考：蛋白純化系列產(chǎn)品-選購指南

產(chǎn)品信息

貨號	36403ES03 / 36403ES05/36403ES08/36403ES25/36403ES60
規(guī)格	1 mL /2 mL/5 mL/25 mL/100 mL

產(chǎn)品性質(zhì)

基質(zhì)（Matrix）	高度交聯(lián)的4%瓊脂糖微球
配體（Ligand）	重組蛋白A/G
孔徑（Bead size）	45-165 µm
最大流速（Flow_max）	0.3 MPa, 3 bar
儲存緩沖液（Buffer）	含20%乙醇的1×PBS
載量（Capacity）	約10-15 mg Rabbit IgG/mL 基質(zhì)
pH范圍（pH range）	3-10

儲存條件

2~8℃保存，有效期2年。

附錄1. 蛋白A，G對不同物種Ig的結(jié)合能力總表

免疫球蛋白亞型	Protein A	Protein G	免疫球蛋白亞型	Protein A	Protein G
Human IgG	++++	++++	Mouse IgG	++++	++++
Human IgG1	++++	++++	Mouse IgG1	+	++++
Human IgG2	++++	++++	Mouse IgG2a	++++	++++
Human IgG3	+	++++	Mouse IgG2b	+++	+++
Human IgG4	++++	++++	Mouse IgG3	++	+++
Human IgM	Use anti-Human IgM		Mouse IgM	Use anti-Mouse IgM
Human IgE	NR	NR	Chicken IgG (IgY)	NR	NR
Human IgA	+	NR	Cow IgG	++	++++
Human IgA1	+	NR	Goat IgG	+	++++
Human IgA2	+	NR	Goat IgG1	+	++++
Human IgD	Use anti-Human IgD		Goat IgG2	++++	++++
Rat IgG	+	++	Goat IgM	NR	NR
Rat IgG1	NR	+	Guinea Pig IgG	++++	++
Rat IgG2a	NR	NR	Guinea Pig IgG1	++++	++
Rat IgG2b	NR	+	Guinea Pig IgG2	++++	++
Rat IgG3	+	++	Hamster IgG	+	++
Sheep IgG	+	++	Horse IgG	+	++++
Sheep IgG1	+	++	Rabbit IgG	++++	+++
Sheep IgG2	+	++	Rabbit IgM	NR	NR
Sheep IgM	NR	NR	Rabbit All isotypes	+++	++
Pig IgG	+++	+++	Monkey IgG	++++	++++
Cat IgG	++++	+	Donkey IgG	++	++++
Dog IgG	++++	+
（+）= weak binding；（++）= moderate binding；（++++）= strong binding；NR= not recommended; （-）= not tested；

Ver.CN20230824

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