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        1. Recombinant Mouse M-CSF Protein,His Tag

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          產(chǎn)品說明書

          FAQ

          COA

          已發(fā)表文獻(xiàn)

           

          Monocyte colony-stimulating factor (M-CSF) is a macrophage lineage-specific growth factor. M-CSF Induces vascular endothelial growth factor production and angiogenic activity from human monocytes. Whatmore, M-CSF and GM-CSF are mediators involved in regulating the numbers and function of macrophage lineage populations and have been shown to contribute to macrophage heterogeneity. 

           

          性能參數(shù)

          Synonyms

          CSF-1, MGI-IM, MCSF, M-CSF, MGC31930

          Uniprot No.

          Q3U4F9

          Source

          Recombinant Mouse M-CSF is expressed from Yeast with N-His. It contains Lys33-Glu262.

          Molecular Weight

          The recombinant mouse CSF1 comprises 230 amino acids and has a predicted molecular mass of 26 kDa. The apparent molecular mass of the protein is approximately 30 kDa in SDS-PAGE under reducing conditions due to glycosylation

          Purity

          > 95% as determined by SDS-PAGE.

          Endotoxin

          <1.0 EU per 1μg of the protein by the LAL method.

          Formulation

          Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4,with 1%BSA.

          Reconstitution

          1. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

          2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

          3.Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options.

           

          儲存條件

          The product should be stored at -25~-15℃ for 1 year from date of receipt.

          2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

          3 months, -25~-15℃ under sterile conditions after reconstitution.

          Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

           

          注意事項(xiàng)

          1.Please operate with lab coats and disposable gloves,for your safety.

          2.This product is for research use only.

           

          產(chǎn)品數(shù)據(jù)

            

          Ver. CN230506

           

          400-6111-883