Q:  使用堿性磷酸酶染色試劑盒（40749ES60），細(xì)胞，本身就是高表達(dá)堿性磷酸酶，做了處理之后可能就稍微低表達(dá)一些，拍出來(lái)的圖，是細(xì)胞一整個(gè)輪廓是染色的？

A: 顏色深淺可以反應(yīng)堿性磷酸酶的多少，但是微量差別可能肉眼無(wú)法觀察，可以進(jìn)行顏色灰度值測(cè)定（此方法可能會(huì)存在結(jié)果不穩(wěn)定問(wèn)題），可參考 WB 條帶的計(jì)算方法。

Q:這個(gè)可以染胚胎干細(xì)胞嗎？

A: 根據(jù)說(shuō)明書中是可以的。本試劑盒可用于細(xì)胞或組織的堿性磷酸酶顯色，包括誘導(dǎo)多功能干細(xì)胞iPS 的鑒定，也可以用于結(jié)合有堿性磷酸酶的膜的 Western 顯色檢測(cè)等。

Q:這個(gè)可以用于判斷細(xì)胞活性不？

A: 不能用于細(xì)胞活性檢測(cè)，本試劑盒主要用于細(xì)胞或組織的堿性磷酸酯酶顯色包括誘導(dǎo)多功能干細(xì)胞 iPS 的鑒定，也可以用于 Western 等結(jié)合有堿性磷酸酯酶的膜的顯色檢測(cè)。同時(shí)也可以用于細(xì)胞或組織內(nèi)源性的堿性磷酸酯酶顯色。 檢測(cè)細(xì)胞活性老師可以用cck-8 等試劑盒。

Q: 使用 24 孔板做成骨細(xì)胞分化后的堿性磷酸酶染色，請(qǐng)問(wèn)可以使用本產(chǎn)品嗎？

A: 可以的。

Q: 配好的堿性磷酸酶工作液可以重復(fù)是使用么，工作液如何保存，保存時(shí)間多久？

A: BCIP/NBT 染色工作液需現(xiàn)配先用，不能重復(fù)使用。

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