问鼎娱乐.com|极速飞艇的冠亚和计划|极速赛车计划群群名|sg幸运飞艇计划官方版下载

<label id="6kt4j"></label>

    <li id="6kt4j"></li>
    1. 

          2. 
    
            
           
           
           
           
          Recombinant Human GDF-7/BMP-12
           
          更多活動(dòng)更多優(yōu)惠，盡在翌圣商城》
           
           
           
          批量采購， 請(qǐng)點(diǎn)擊詢價(jià)
           
           
          產(chǎn)品說明書
                  
          FAQ
                  
          COA
                  
          已發(fā)表文獻(xiàn)
                  
           
          Growth Differentiation Factor-7 (GDF-7; also called BMP-12 and CDMP-3) is a member of the BMP family of TGF-beta superfamily proteins. GDF­7 is synthesized as a large precursor protein that consists of an N-­terminal 19 amino acid (aa) signal sequence, a 302 aa pro region and a 129 aa C-terminal mature peptide. At the amino acid level, mature human GDF-7 shares 85% and 88% aa sequence identity with mature GDF-7 in mouse and rat, respectively. Mature human GDF-7 lacks a glycine repeat that is found in both mouse and rat GDF-7. Based on sequence similarity, GDF-7 is categorized with GDF-5 and -6, as a subgroup within the BMP family. GDF-7 functions as a homodimer and elicits its bioactivity by mediating the formation of a heterodimeric receptor complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor. GDF-7 signaling results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8). 
          


           
          


          Product Properties
          


          
	
		
          
			
			
          Synonyms
          
			          		
			
			
          BMP12，GDF7
          
			          		
		
          
		
          
			
			
          Accession
          
			          		
			
			
          Q7Z4P5
          
			          		
		
          
		
          
			
			
          GeneID
          
			          		
			
			
          151449
          
			          		
		
          
		
          
			
			
          Source
          
			          		
			
			
          E.coli-derived Human GDF-7/BMP-12, Thr322-Arg450.
          
			          		
		
          
		
          
			
			
          Molecular Weight
          
			          		
			
			
          Approximately 28.0 kDa.
          
			          		
		
          
		
          
			
			
          AA Sequence
          
			          		
			
			
          TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR
          
			          		
		
          
		
          
			
			
          Tag
          
			          		
			
			
          None
          
			          		
		
          
		
          
			
			
          Physical Appearance
          
			          		
			
			
          Sterile Filtered White lyophilized (freeze-dried) powder.
          
			          		
		
          
		
          
			
			
          Purity
          
			          		
			
			
          > 95% by SDS-PAGE and HPLC analyses.
          
			          		
		
          
		
          
			
			
          Biological Activity
          
			          		
			
			
           The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/mL, corresponding to a specific activity of > 1000 IU/mg. Fully biologically active when compared to standard. 
          
			          		
		
          
		
          
			
			
          Endotoxin
          
			          		
			
			
          < 0.1 EU per 1μg of the protein by the LAL method.
          
			          		
		
          
		
          
			
			
          Formulation
          
			          		
			
			
          Lyophilized from a 0.2 µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.
          
			          		
		
          
		
          
			
			
          Reconstitution
          
			          		
			
			
          We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
          
			          		
		
          
	          

          


           
          


          Shipping and Storage
          


          The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
          


          Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
          


           
          


          Cautions
          


          1. Avoid repeated freeze-thaw cycles.
          


          2. For your safety and health, please wear lab coats and disposable gloves for operation.
          


          3. For research use only!
          


           
          


          HB220318
          


           
          

             
           
           
           
           
           
           
           
          400-6111-883