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          首頁 / 建模實驗 / 產品應用詳情
           
          DSS誘導果蠅潰瘍性結腸炎模型的建立
           
          葡聚糖硫酸鈉(Dextran Sulfate Sodium，DSS)是葡聚糖的聚陰離子衍生物，喂食果蠅會破壞腸上皮中腸道表皮細胞之間的連接從而造成腸道損傷，引起多條通路應答調控干細胞的自我更新及分化，補充受損細胞，維持腸道穩(wěn)態(tài)。  
          


          1.png 
          


          圖1. 果蠅腸道干細胞分裂分化示意圖
          


           
          


          

          

          

          實驗動物
          

          

          

          


          5-10日齡果蠅，雌性 
          


           
          


          

          

          

          實驗材料
          

          

          

          


          DSS（yeasen DSS 60316ES,MW36000-50000） 
          


           
          


          

          

          

          實驗步驟
          

          

          

          


          1. 用5%的蔗糖溶液配制喂食培養(yǎng)基；
          
2. 對照組：5%蔗糖培養(yǎng)基（SC），實驗組：3% DSS（培養(yǎng)基配制）；
          
3. 預先用500 μL的喂食培養(yǎng)基將2.5cm×3.75cm的層析紙潤濕，然后放入空的培養(yǎng)瓶中；
          
4. 果蠅放入含有層析紙的瓶中，29℃培養(yǎng)三天，期間每天將存活的果蠅移入含有新鮮培養(yǎng)基的空瓶中；
          
5. 培養(yǎng)三天后，切開中腸，用GFP（綠色）和PH3（紅色）抗體和DRAQ5（藍色）進行免疫染色。
          


           
          


          

          

          

          

          實驗結果
          

          

          

          

          


          2.png
          
圖2.  DSS誘導果蠅中ISC前體細胞增殖[1]
          


           通過免疫染色中不同的標記方法，量化了腸道干細胞（ISC），ISC前體細胞發(fā)生增值，表明DSS誘導果蠅模型腸道發(fā)生損傷。
          


           
          


          

          

          

          

          

          

          翌圣DSS產品優(yōu)勢
          

          

          

          

          

          

          


          翌圣生物提供高品質DSS（Cat.NO：60316ES，MW:36000~50000），具有以下優(yōu)勢：
          


          1. 高純度（＞98%），硫含量17-19%，游離硫＜0.2%，大量數據文獻支持，廣泛應用于UC模型的構建。
          


          2. 多種屬動物中均可造模：小鼠、大鼠、斑馬魚、豬、果蠅；
          


          3. 癥狀表現(xiàn)與人UC極度相似：可用于研究急、慢性結腸炎的發(fā)生發(fā)展機制，也可用于藥物的藥效研究。
          


          4. 簡單易行，成模率高，重復性強
          


          5. 聯(lián)合氧化偶氮甲烷 (azoxymethane, AOM)用藥，可用于誘發(fā)結腸炎相關性癌癥 (colitis associated cancer, CAC) 動物模型，成功模擬IBD誘發(fā)CAC的過程。 
          


          3.png
          


          建模效率與進口產品一致，產品價格低至進口價格的1/3，大量現(xiàn)貨庫存。
          


           
          


          

          

          

          

          

          

          產品訂購
          

          

          

          

          

          

          


          
	
		
          
			
			
          產品名稱
          
			          		
			
			
          貨號
          
			          		
			
			
          規(guī)格
          
			          		
		
          
		
          
			
			
          ColitCare® Dextran Sulfate Sodium Salt（DSS）
          

			
          結腸炎建模用葡聚糖硫酸鈉鹽 MW : 36000～50000
          
			          		
			
			
          60316ES25
          
			          		
			
			
          25 g
          
			          		
		
          
		
          
		
          
		
          
			
			
          ColitCare® Dextran Sulfate Sodium Salt（DSS）
          

			
          結腸炎建模用葡聚糖硫酸鈉鹽 MW : 36000～50000
          
			          		
			
			
          60316ES60
          
			          		
			
			
          100 g
          
			          		
		
          
		
          
		
          
		
          
			
			
          ColitCare® Dextran Sulfate Sodium Salt（DSS）
          

			
          結腸炎建模用葡聚糖硫酸鈉鹽 MW : 36000～50000
          
			          		
			
			
          60316ES76
          
			          		
			
			
          500 g
          
			          		
		
          
		
          
		
          
		
          
			
			
          ColitCare® Dextran Sulfate Sodium Salt（DSS）
          

			
          結腸炎建模用葡聚糖硫酸鈉鹽 MW : 36000～50000
          
			          		
			
			
          60316ES80
          
			          		
			
			
          1 kg
          
			          		
		
          
		
          
		
          
	          

          


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          產品已發(fā)表文章(部分)
          

          

          

          

          

          

          


          向下滑動查看
          


           
          


          

          

          

          2022
          


          [1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717
          


          [2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143
          


          [3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451
          


          [4] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076
          


          [5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869
          


           
          


          2021
          


          [1] Li Zhao, Fei Wang , Zhengwei Cai，et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].Chemical Engineering Journal.424(2021)130464. IF=16.744
          


          [2] Hao H,  Zhang X,  Tong L,  Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561
          


          [3] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717
          


          [4] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943
          


          [5] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
          


          [6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561
          


           
          


          2020
          


          [1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561
          


          [2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048
          


          [3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
          


           
          


          BEFORE 2020
          


          [1] Oehlers SH, Flores MV, Hall CJ, Crosier KE, Crosier PS. Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis. Dis Model Mech. 2012 Jul;5(4):457-67.IF=4.973
          


          [2] Kim CJ, Kovacs-Nolan JA, Yang C, Archbold T, Fan MZ, Mine Y. l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Nutr Biochem . 2010 Jun;21(6):468-75.IF=6.048
          


          [3] Karpowicz , P., Perez, J. & Perrimon , N.,. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration. Development (Cambridge, England), 2010,137(24), pp.4135–4145.IF=6.868
          


          [4] Fan H, Chen W, Zhu J, et al. Toosendanin alleviates dextran sulfate sodium-induced colitis by inhibiting M1 macrophage polarization and regulating NLRP3 inflflammasome and Nrf2/HO-1 signaling[J]. International immunopharmacology, 2019, 76: 105909.IF=3.943
          

          

          

          


           
          


          

          

          

          

          

          往期精彩
          

          

          

          


          

           
          

          


          

          1.不同分子量葡聚糖硫酸鈉選購指南：高品質DSS葡聚糖硫酸鈉鹽
          


          2.DSS結腸炎造模整體解決方案：Dextran Sulfate Sodium Salt（DSS） 潰瘍性結腸炎模型的建立 (yeasen.com) 
          

          

          

          


           
          


          

          

          

          

          參考文獻
          

          

          

          

          


          [1] Fangfang Ren,et al.PNAS.2010.107(49) 21064-21069
          

           
           
           
           
           
           
           
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