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        1. Recombinant Mouse RANKL/TNFSF11/CD254 Protein,His Tag

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          產(chǎn)品說明書

          FAQ

          COA

          已發(fā)表文獻(xiàn)

           

          性能參數(shù)

          分子別名(Synonyms)

          ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2

          表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)

          Mouse RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr70-Asp316.[Accession | AAC40113.1]

          分子量大?。∕olecular Weight)

          The protein has a predicted MW of 28.97 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on SDS-PAGE result.

          內(nèi)毒素(Endotoxin)

          Less than 1EU per μg by the LAL method.

          純度(Purity)

          > 95% as determined by SDS-PAGE and HPLC.

          活性(Activity)

          ELISA Data: Immobilized Mouse RANKL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.

          制劑(Formulation)

          Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

          外觀(Appearance)

          凍干粉

           

          儲(chǔ)存條件

          -25~-15℃保存,收到貨之后有效期1年。復(fù)溶后, -85~-65℃保存,3個(gè)月有效期。

          復(fù)溶方法

          開蓋之前,先離心。使用蒸餾水復(fù)溶凍干的蛋白質(zhì),復(fù)溶濃度超過100 μg/mL。建議首次使用時(shí)將蛋白分裝為每次實(shí)驗(yàn)用量,避免反復(fù)凍融。

          注意事項(xiàng)

          1.為了您的安全和健康,請穿實(shí)驗(yàn)服并戴一次性手套操作。

          2.本產(chǎn)品僅作科研用途。    

          400-6111-883