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        1. Recombinant PE-Labeled Human Peptide Ready HLA-A*02:01&B2M TetramerProtein,His-Avi Tag

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          產(chǎn)品說明書

          FAQ

          COA

          已發(fā)表文獻(xiàn)

           

          性能參數(shù)

          分子別名(Synonyms)

          MHC; HLA-A*02:01; Peptide Ready

          表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)

          PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidin It contains Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M).[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)]

          內(nèi)毒素(Endotoxin)

          Less than 1EU per μg by the LAL method.

          活性(Activity)

          ELISA Data: Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

          制劑(Formulation)

          Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

          重構(gòu)方法(Reconstitution)

          Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

           

          儲(chǔ)存條件

          1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

          2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

          3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

          4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

           

          注意事項(xiàng)

          1.Please operate with lab coats and disposable gloves,for your safety.

          2.This product is for research use only.

          400-6111-883