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        1. Recombinant Cynomolgus IL-4 Protein,His Tag

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          產(chǎn)品說明書

          FAQ

          COA

          已發(fā)表文獻

           

          性能參數(shù)

          分子別名(Synonyms)

          IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1

          表達區(qū)間及表達系統(tǒng)(Source)

          Cynomolgus IL-4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains His25-Ser153.[Accession | P79339-1]

          分子量大?。∕olecular Weight)

          The protein has a predicted MW of 16.03 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on SDS-PAGE result.

          內(nèi)毒素(Endotoxin)

          Less than 1EU per μg by the LAL method.

          純度(Purity)

          > 95% as determined by SDS-PAGE and HPLC.

          活性(Activity)

          SPR Data:Cynomolgus IL-4, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay .

          制劑(Formulation)

          Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

          重構(gòu)方法(Reconstitution)

          Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

           

          儲存條件

          1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

          2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

          3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

          4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

           

          注意事項

          1.Please operate with lab coats and disposable gloves,for your safety.

          2.This product is for research use only.

          400-6111-883