The CRISPR associated protein 9 (Cas9) is an RNA-guided DNA nuclease and part of the Streptococcus pyogenes CRISPR antiviral immunity system that provides adaptive immunity against extra chromosomal genetic material. CRISPR/Cas9 genome editing tools have been used in many organisms, including mouse and human cells.

 

產(chǎn)品性質(zhì)

別名	Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9
SwissProt ID	Q99ZW2
免疫原	A synthesized peptide derived from CRISPR-Cas9 SP.
緩沖液	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
分子量	Calculated MW: 158 kDa; Observed MW: 158 kDa.
克隆類型	Polyclonal Antibody
亞型	IgG
宿主	Rabbit
物種反應(yīng)	Streptococcus pyogenes
推薦應(yīng)用	WB, IHC-P, ICC/IF, FC
純化方式	Affinity Purified

 

應(yīng)用

稀釋比 WB:1/500-1/1000; IHC: 1/50-1/100; IF:1/50-1/200; FC:1/50-1/100

 

儲(chǔ)存條件

-25~-15℃保存

 

注意事項(xiàng)

1. 避免反復(fù)凍融。

2. 本產(chǎn)品僅作科研用途。

3. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并佩戴一次性手套操作。

 

產(chǎn)品數(shù)據(jù)

Western blot

Western blot analysis of CRISPR Cas9 in (1) 293T lysates transfected with CRISPR Cas9; (2) 293T lysates; (3) 3T3 lysates; (4) PC-12 lysates using CRISPR Cas9 antibody.

Immunofluorescence

Immunofluorescence analysis of CRISPR Cas9 in 293T transfected with CRISPR Cas9 using CRISPR Cas9 antibody.

 

Ver.CN20231214