KIT encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.

 

產(chǎn)品性質(zhì)

別名	KIT; SCFR; Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD antigen CD117
SwissProt ID	P10721
基因ID	3815
免疫原	The antiserum was produced against synthesized peptide derived from human c-Kit.
緩沖液	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
分子量	Calculated MW: 110 kDa; Observed MW: 120 kDa
克隆類(lèi)型	Polyclonal Antibody
宿主	Rabbit
物種反應(yīng)	Human, Mouse, Rat, Monkey
推薦應(yīng)用	WB, IHC-F, IHC-P, ICC/IF, ELISA
純化方式	Affinity Purified

 

應(yīng)用

稀釋比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/50-1/200; ELISA: 1/10000

 

儲(chǔ)存條件

-25~-15℃保存

 

注意事項(xiàng)

1. 避免反復(fù)凍融。

2. 本產(chǎn)品僅作科研用途。

3. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并佩戴一次性手套操作。

 

產(chǎn)品數(shù)據(jù)

Western blot

Western blot analysis of c-Kit in COS7/A549 lysates using c-Kit antibody. The lane on the right is blocked with the synthesized peptide.

 

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human Mammary cancer using c-Kit antibody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.

 

Immunohistochemistry analysis of paraffin-embedded Human tonsils using c-Kit antibody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.

 

Immunohistochemistry analysis of paraffin-embedded Human Mammary cancer using c-Kit antibody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.

 

 

Ver.CN20231026