表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human IL-18 R1/CD218a Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala19-Arg329.[Accession | AAH69575]

分子量大?。?/font>Molecular Weight）

The protein has a predicted MW of 38.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

SPR Data: Biotinylated Human IL-18 R1, His Tag captured on CM5-SA Chip can bind Human IL-18, No Tag with an affinity constant of 1.4nM as determined in SPR assay

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.