表達區(qū)間及表達系統(tǒng)（Source）

Human TRAIL R4/TNFRSF10D Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala56-His211.[Accession | Q9UBN6]

分子量大小（Molecular Weight）

The protein has a predicted MW of 19.4 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human TRAIL, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R4, His Tag with the EC50 of 76.1ng/ml determined by ELISA.  

SPR Data: Human TRAIL R4, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.13 nM as determined in SPR assay 

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.