表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-15RA&IL-15 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).[Accession | Q13261-1&P40933-1]

分子量大?。?/font>Molecular Weight）

The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per ug by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human IL-15RA&IL-15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 72.0ng/ml determined by ELISA  

SPR Data: Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.