表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.

It contains Gly17-Gln208(V176).[Uniprot | AAH17865]

分子量大?。?/font>Molecular Weight）

The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and  HPLC

活性（Activity）

ELISA Data: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 2ng/ml determined by ELISA.

SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIIA (V176) , His-Avi Tag with an affinity constant of 0.539 μM as determined in SPR assay

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100

μg/ml is recommended. Dissolve the lyophilized protein in distilled water.