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        1. Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein, His-Avi Tag

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          產(chǎn)品說明書

          FAQ

          COA

          已發(fā)表文獻

           

          Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified.

           

          產(chǎn)品性質(zhì)

          Synonyms

          CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10

          Accession

          O75015-1

          Source

          Recombinant Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Ser200. The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106. The CD16b NA1form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36, S65, N82, and I106.

          Molecular Weight

          The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Tris-Bis PAGE result.

          Physical Appearance

          Sterile Filtered White lyophilized (freeze-dried) powder.

          Purity

          > 95% as determined by SDS-PAGE and HPLC

          Endotoxin

          1.0 EU per 1μg of the protein by the LAL method.

          Formulation

          Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

          Reconstitution

          Centrifuge tubes before opening. Reconstituting to a concentration more than 100μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

           

          運輸與保存方法

          The products are shipped with ice pack and can be stored at -20for 1 year.

          2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
          3 -6 months, -20 to -80°C under sterile conditions after reconstitution.

          Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

           

          注意事項

          1. For your safety and health, please wear lab coats and disposable gloves for operation.
          2. For research use only!
          3.  

          產(chǎn)品數(shù)據(jù)

          HB230208

          400-6111-883