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          Recombinant SARS-COV-2 Spike RBD(Omicron XBB) Protein,  His Tag
           
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          產(chǎn)品說明書
                  
          FAQ
                  
          COA
                  
          已發(fā)表文獻(xiàn)
                  
           
           
          


          Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) . The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 . The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2).
          


           
          


          產(chǎn)品信息
          


          
	
		
          
			
			
          別名
          
			          		
			
			
          S protein RBD; S glycoprotein RBD; Spike protein RBD
          
			          		
		
          
		
          
			
			
          Accession
          
			          		
			
			
          QHD43416.1
          
			          		
		
          
		
          
			
			
          表達(dá)區(qū)間及表達(dá)系統(tǒng)
          
			          		
			
			
          Recombinant SARS-CoV-2 Spike RBD (Omicron XBB) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H).
          
			          		
		
          
		
          
			
			
          分子量
          
			          		
			
			
          Approximately 26.21 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
          
			          		
		
          
		
          
			
			
          純度
          
			          		
			
			
          > 95% as determined by SDS-PAGE and HPLC.
          
			          		
		
          
		
          
			
			
          活性
          
			          		
			
			
          ELISA Data：Immobilized SARS-CoV-2 Spike RBD (Omicron XBB) at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
          
			          		
		
          
		
          
			
			
          內(nèi)毒素
          
			          		
			
			
          < 1.0 EU per 1μg of the protein by the LAL method.
          
			          		
		
          
		
          
			
			
          制劑
          
			          		
			
			
          Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). 
          
			          		
		
          
		
          
			
			
          復(fù)溶
          
			          		
			
			
          Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
          
			          		
		
          
	          

          


           
          


          儲存條件
          


          The product should be stored at -25~-15℃.
          


          1 month, 2 ~8 °C under sterile conditions after reconstitution.
          


          3 months, -25~-15℃ °C under sterile conditions after reconstitution.
          


          Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
          


           
          


          注意事項
          


          1.Please operate with lab coats and disposable gloves，for your safety。
          


          2.This product is for research use only. 
          


           
          

             
           
           
           
           
           
           
           
          400-6111-883