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          Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
           
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          產(chǎn)品說(shuō)明書
                  
          FAQ
                  
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          This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.
          


          This product is produced in accordance with GMP process requirements, and the product is provided in liquid form
          


           
          


          Product Properties
          


          
	
		
          
			
			
          Source
          
			          		
			
			
          Recombinant E.coli with Pyrophosphatase gene from yeast
          
			          		
		
          
		
          
			
			
          Optimum Temperature
          
			          		
			
			
          25℃
          
			          		
		
          
		
          
			
			
          Storage Buffer
          
			          		
			
			
          50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% （v/v）TritonX-100, 50%（v/v）glycerin
          
			          		
		
          
		
          
			
			
          Unit Definition
          
			          		
			
			
          One unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.（The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min）
          
			          		
		
          
	          

          


           
          


          Contents
          


          
	
		
          
			
			
          Catalog No.
          
			          		
			
			
          Name
          
			          		
			
			
          Catalog No./Specification
          
			          		
		
          
		
          
			
			
          10672ES03
          

			
          (1 U)
          
			          		
			
			
          10672ES10
          

			
          (10 U)
          
			          		
			
			
          10672ES60
          

			
          (100 U)
          
			          		
		
          
		
          
			
			
          10672
          
			          		
			
			
          Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
          
			          		
			
			
          10 μL
          
			          		
			
			
          100 μL
          
			          		
			
			
          1 mL
          
			          		
		
          
	          

          


           
          


          Shipping and Storage
          


          The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
          


           
          


          Notes
          


          1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.
          


          2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.
          


          3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this
          


           
          


          HB220701
          

             
           
           
           
           
           
           
           
          400-6111-883