產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a 130-155 kDa member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Within ECD human Flt-3 shares 85% and 82% aa sequence identity with mouse and rat Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations.
產(chǎn)品性質(zhì)
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別名（Synonyms） |
CD135, FLK2, STK-1, FLT-3 |
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Uniprot No. |
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表達區(qū)間及表達系統(tǒng)（Source） |
Recombinant Human FLT3 / Flk-2 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Asn27-Asn541. |
|
分子量(Molecular Weight) |
Approximately 61.2 kDa. Due to glycosylation, the protein migrates to 80-105 kDa based on Tris-Bis PAGE result. |
|
外觀(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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純度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Human FLT3, His Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 0.1 μg/mL determined by ELISA. |
|
內(nèi)毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制劑（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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復溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運輸與保存方法
冰袋運輸。-20℃至-80℃保存，一年有效期。
復溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復溶后，2-8°C 保存 2-7 天。
建議第一次使用時分裝凍存，避免反復凍融。
注意事項
1. 避免反復凍融。
2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
3. 本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
Tris-Bis PAGE
HB220707
