產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻(xiàn)
VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.
產(chǎn)品性質(zhì)
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別名（Synonyms） |
CD309, KDR, VEGFR, VEGFR2, VEGFR-21, FLK1, KRD1, Ly73 |
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Uniprot No. |
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表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） |
Recombinant Human VEGF R2/KDR Protein is expressed from HEK293 Cells with mFc tag at the C-terminal. It contains Ala20-Glu764. |
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分子量(Molecular Weight) |
Approximately 110 kDa. Due to glycosylation, the protein migrates to 150-200 kDa based on Tris-Bis PAGE result. |
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外觀(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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純度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
ELISA Data: Immobilized Human VEGF165 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC50 of 36.1 ng/mL determined by ELISA. |
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內(nèi)毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制劑（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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復(fù)溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃至-80℃保存，一年有效期。
復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后，2-8°C 保存 2-7 天。
建議第一次使用時(shí)分裝凍存，避免反復(fù)凍融。
注意事項(xiàng)
1. 避免反復(fù)凍融。
2. 為了您的安全和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。
3. 本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
Tris-Bis PAGE
HB220630
