產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻
HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity. Mature human HVEM consists of a 164 aa extracellular domain (ECD) with three cysteine-rich domains (CRD), a 21 aa transmembrane segment, and a 60 aa cytoplasmic tail with a TRAF interaction domain. Within the ECD, human HVEM shares 55% aa sequence identity with mouse and rat HVEM. Alternative splicing generates an additional isoform with a substitution of the N-terminal 10 amino acids including the signal peptide. HVEM is highly expressed on naïve CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils. Its expression declines during effector T cell activation but is up-regulated during Treg activation. HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha.
產(chǎn)品性質
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別名（Synonyms） |
CD270; CD40-like protein; HVEM; LIGHTR; ATAR; TNFRSF14; TR2 |
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Uniprot No. |
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表達區(qū)間及表達系統(tǒng)（Source） |
Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Leu39-Val202. |
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分子量(Molecular Weight) |
The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result. |
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外觀(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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純度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
1.ELISA Data：Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 19.9ng/ml determined by ELISA. 2.ELISA Data：Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.44μg/ml determined by ELISA. 3.ELISA Data：Immobilized Human HVEM, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. |
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內(nèi)毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制劑（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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復溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運輸與保存方法
冰袋運輸。-20℃至-80℃保存，一年有效期。
復溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復溶后，2-8°C 保存 2-7 天。
建議第一次使用時分裝凍存，避免反復凍融。
注意事項
1. 避免反復凍融。
2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
3. 本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
Tris-Bis PAGE
HB220624
