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          Recombinant Human LIGHT/TNFSF14(His Tag)
           
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          產(chǎn)品信息
          


          
	
		
          
			
			
          產(chǎn)品名稱
          
			          		
			
			
          產(chǎn)品編號
          
			          		
			
			
          規(guī)格
          
			          		
		
          
		
          
			
			
          Recombinant Human LIGHT / TNFSF14 
          
			          		
			
			
          93747ES25
          
			          		
			
			
          25 μg
          
			          		
		
          
		
          
			
			
          93747ES60
          
			          		
			
			
          100 μg
          
			          		
		
          
		
          
			
			
          93747ES76
          
			          		
			
			
          500 μg
          
			          		
		
          
	          

          


           
          


          產(chǎn)品描述
          


          LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.
          


           
          


          產(chǎn)品性質(zhì)
          


          
	
		
          
			
			
          別名（Synonyms）
          
			          		
			
			
          CD258, TNFSF14, HVEM-L, LIGHT, LTg, TR2
          
			          		
		
          
		
          
			
			
          Uniprot No.
          
			          		
			
			
          O43557-1
          
			          		
		
          
		
          
			
			
          表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
          
			          		
			
			
          HEK293 Cells-derived Human LIGHT / TNFSF14 Protein, Asp74-Val240 with hFc tag at the N-terminal
          
			          		
		
          
		
          
			
			
          分子量(Molecular Weight)
          
			          		
			
			
          Approximately 19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
          
			          		
		
          
		
          
			
			
          外觀(Physical Appearance）
          
			          		
			
			
          Sterile Filtered White lyophilized (freeze-dried) powder.
          
			          		
		
          
		
          
			
			
          純度（Purity）
          
			          		
			
			
          > 95% as determined by SDS-PAGE and HPLC
          
			          		
		
          
		
          
			
			
          活性（Activity）
          
			          		
			
			
          1.ELISA Data: Immobilized Human LIGHT, His Tag at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 0.12 μg/mL determined by ELISA.
          

			
          2.ELISA Data: Immobilized Human LIGHT, His Tag at 0.5 μg/ml (100 μL/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 of 22.9 ng/mL determined by ELISA.
          
			          		
		
          
		
          
			
			
          內(nèi)毒素（Endotoxin）
          
			          		
			
			
          < 1.0 EU per 1μg of the protein by the LAL method.
          
			          		
		
          
		
          
			
			
          制劑（Formulation）
          
			          		
			
			
          Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
          
			          		
		
          
		
          
			
			
          復(fù)溶（Reconstitution）
          
			          		
			
			
          Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
          
			          		
		
          
	          

          


           
          


          運輸與保存方法
          


          冰袋運輸。-20℃至-80℃保存，一年有效期。
          


          復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復(fù)溶后，2-8°C 保存 2-7 天。
          

          


          

          建議第一次使用時分裝凍存，避免反復(fù)凍融。
          


          

           
          


          注意事項
          


            
	
          1. 避免反復(fù)凍融。
            2. 
	
          2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
            3. 
	
          3. 本產(chǎn)品僅作科研用途！
            4. 

          


           
          


          產(chǎn)品數(shù)據(jù)
          


          Tris-Bis PAGE
          


          


           
          


          SEC-HPLC
          


          

          


          ELISA Data
          


           
          


           
          


          ELISA Data
          


           
          

          

             
           
           
           
           
           
           
           
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