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          Recombinant Human TGF-alpha Protein
           
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          產(chǎn)品說明書
                  
          FAQ
                  
          COA
                  
          已發(fā)表文獻(xiàn)
                  
           
          Transforming Growth Factor-alpha (TGF-α) , also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines.  TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells.Mature TGF-alpha  shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.
          


           
          


          Product Properties
          


          
	
		
          
			
			
          Synonyms
          
			          		
			
			
          Transforming Growth Factor alpha; TGF-a; TGF-type I; ETGF
          
			          		
		
          
		
          
			
			
          Accession
          
			          		
			
			
          P01135
          
			          		
		
          
		
          
			
			
          Gene ID
          
			          		
			
			
          7039
          
			          		
		
          
		
          
			
			
          Source
          
			          		
			
			
          E.coli-derived Human TGF-a protein, Vla40-Ala89 
          
			          		
		
          
		
          
			
			
          Molecular Weight
          
			          		
			
			
          Approximately 5.8 kDa, on SDS-PAGE under reducing conditions.
          
			          		
		
          
		
          
			
			
          AA Sequence
          
			          		
			
			
          V VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLA
          
			          		
		
          
		
          
			
			
          Tag
          
			          		
			
			
          None
          
			          		
		
          
		
          
			
			
          Physical Appearance
          
			          		
			
			
          Sterile Filtered White lyophilized (freeze-dried) powder.
          
			          		
		
          
		
          
			
			
          Purity
          
			          		
			
			
          > 95% as determined by SDS-PAGE
          
			          		
		
          
		
          
			
			
          Biological Activity
          
			          		
			
			
          Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is < 0.4 ng/mL.
          
			          		
		
          
		
          
			
			
          Endotoxin
          
			          		
			
			
          < 1.0 EU per 1μg of the protein by the LAL method.
          
			          		
		
          
		
          
			
			
          Formulation
          
			          		
			
			
          Lyophilized after extensive dialysis against PBS.
          
			          		
		
          
		
          
			
			
          Reconstitution
          
			          		
			
			
          We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
          
			          		
		
          
	          

          


           
          


          Shipping and Storage
          


          The products are shipped with ice pack and can be stored at -80℃ for 1 year from date of receipt.
          
1week, 2 to 8 °C under sterile conditions after reconstitution.
          
3 months, -20 °C under sterile conditions after reconstitution.
          


          Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
          


           
          


          Cautions
          


          1.For your safety and health, please wear lab coats and disposable gloves for operation.
          


          2.For research use only!
          


          HB230921
          

             
           
           
           
           
           
           
           
          400-6111-883