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        1. Recombinant Rat SDF-1α/CXCL12α Protein

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          產(chǎn)品說明書

          FAQ

          COA

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          CXCL12 is belonging to the CXC chemokine family. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α.

           

          Product Properties

          Synonyms

          CXCL12 chemokine (C-X-C motif) ligand 12, CXCL12/SDF-1 alpha, IRH, PBSF, SCYB12, SDF1 alpha,  SDF1, SDF1a, SDF1B

          Accession

          Q9QZD1

          GeneID

          24772

          Source

          E.coli-derived rat SDF-1α/CXCL12α protein, Lys22-Lys89.

          Molecular Weight

          Approximately 7.9 kDa.

          AA Sequence

          KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK

          Tag

          None

          Physical Appearance

          Sterile Filtered White lyophilized (freeze-dried) powder.

          Purity

          >97% by SDS-PAGE and HPLC analyses.

          Biological Activity

          The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/mL. Fully biologically active when compared to standard.

          Endotoxin

          < 1.0 EU per 1μg of the protein by the LAL method.

          Formulation

          Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

          Reconstitution

          We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

           

          Shipping and Storage

          The products are shipped with ice pack and can be stored at -20 for 1 year.

          1 month, 2 to 8 °C under sterile conditions after reconstitution.

          3 months, -20 °C under sterile conditions after reconstitution.

          Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

           

          Cautions

          1.For your safety and health, please wear lab coats and disposable gloves for operation.

          2.For research use only!

          HB221111

          400-6111-883