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          Recombinant Human NT-3
           
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          產(chǎn)品說明書
                  
          FAQ
                  
          COA
                  
          已發(fā)表文獻(xiàn)
                  
           
          Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.
          


           
          


          Product Properties
          


          
	
		
          
			
			
          Synonyms
          
			          		
			
			
          HDNF ; NGF-2 ; NGF2 ; NT-3; NT3
          
			          		
		
          
		
          
			
			
          Accession
          
			          		
			
			
          P20783-1
          
			          		
		
          
		
          
			
			
          Source
          
			          		
			
			
          E. coli-derived human NT-3 protein, Tyr139-Thr257 
          
			          		
		
          
		
          
			
			
          Molecular Weight
          
			          		
			
			
          Approximately 13.8 kDa. 
          
			          		
		
          
		
          
			
			
          AA Sequence
          
			          		
			
			
          YA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK TGNSPVKQYF YETRCKEARP 
          

			
          VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI RIDTSCVCAL SRKIGRT 
          
			          		
		
          
		
          
			
			
          Tag
          
			          		
			
			
          None
          
			          		
		
          
		
          
			
			
          Physical Appearance
          
			          		
			
			
          Sterile Filtered White lyophilized (freeze-dried) powder.
          
			          		
		
          
		
          
			
			
          Purity
          
			          		
			
			
          > 90% by SDS-PAGE.
          
			          		
		
          
		
          
			
			
          Biological Activity
          
			          		
			
			
          Measured by its binding ability in a functional ELISA. Immobilized human NT3 at 2 μg/mL (100 μL/well) can bind human TrkB-Fch. The EC50 of human TrkB-Fch is 120-350 ng/mL.
          
			          		
		
          
		
          
			
			
          Endotoxin
          
			          		
			
			
          < 1.0 EU per 1μg of the protein by the LAL method.
          
			          		
		
          
		
          
			
			
          Formulation
          
			          		
			
			
          Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.2. 
          
			          		
		
          
		
          
			
			
          Reconstitution
          
			          		
			
			
          We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
          
			          		
		
          
	          

          


           
          


          Shipping and Storage
          


          The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
          


          Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
          


           
          


          Cautions
          


          1. Avoid repeated freeze-thaw cycles.
          


          2. For your safety and health, please wear lab coats and disposable gloves for operation.
          


          3. For research use only.
          


            
          


          HB220415
          

             
           
           
           
           
           
           
           
          400-6111-883