Hieff NGS? G-Type In-Situ DNA Binding Profiling Library Prep Kit for Illumina? CUT&Tag建庫試劑盒
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產(chǎn)品信息
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產(chǎn)品名稱 |
產(chǎn)品編號 |
規(guī)格 |
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Hieff NGS® G-Type In-Situ DNA Binding Profiling Library Prep Kit for Illumina® CUT&Tag試劑盒（pA/G-Tn5版） |
12598ES04 |
4 T |
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12598ES12 |
12 T |
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12598ES48 |
48 T |
組分信息
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組分編號 |
組分名稱 |
12598ES04 |
12598ES12 |
12598ES48 |
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BOX-I |
12598-A |
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ConA Beads |
40 μL |
120 μL |
480 μL |
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12598-B |
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DNA Selection Beads |
400 μL |
1.2 mL |
4.8 mL |
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12598-C |
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Cell wash buffer |
3.6 mL |
10.8 mL |
43.2 mL |
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12598-D |
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ConA bind buffer |
400 μL |
1.2 mL |
4.8 mL |
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BOX-II |
12598-E |
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Protease Inhibitor Cocktail (EDTA-free) |
40 μL |
120 μL |
480 μL |
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12598-F |
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5% Digitonin |
15 μL |
45 μL |
180 μL |
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12598-G |
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3 M NaCl |
80 μL |
240 μL |
960 μL |
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12598-H |
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50x Protein blocker |
4 μL |
12 μL |
48 μL |
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12598-I |
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pA/G-Transposome Mix |
4 μL |
12 μL |
48 μL |
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12598-J |
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30x Activating buffer |
4 μL |
12 μL |
48 μL |
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12598-K |
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15x Terminate Solution |
8 μL |
24 μL |
96 μL |
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12598-L |
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DNA Spike-in mix (5 pg/uL) |
4 μL |
12 μL |
48 μL |
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12598-M |
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30x Proteinase K |
4 μL |
12 μL |
48 μL |
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12598-N |
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Primer Mix |
12 μL |
36 μL |
144 μL |
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12598-O |
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2×Ultima Amplification Mix |
100 μL |
300 μL |
1.2 mL |
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12598-P |
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N5(N501) * |
4 μL |
NA |
NA |
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12598-Q |
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N7(N701) * |
4 μL |
NA |
NA |
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【注】：*測序時 Index 序列 填寫時，測序平臺為NovaSeq 6000 v1.0 reagents, MiSeq, HiSeq 2000/2500，則N501-CTCTCTAT，N701-TAAGGCGA。測序平臺為NovaSeq 6000 v1.5 reagents，MiniSeq, NextSeq,HiSeq 3000/4000，則N501-ATAGAGAG，N701-TAAGGCGA。
儲存條件
BOX-I：2-8℃保存，BOX-II：-25~-15℃保存，有效期1年。
CN20231208
