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        1. Recombinant Mouse R spondin 1/RSPO1 Protein,His Tag(HEK293) 重組小鼠RSPO1蛋白

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          產(chǎn)品說明書

          FAQ

          COA

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          產(chǎn)品描述

           

          R-Spondin-1(Rspo-1)屬于Wnt調(diào)節(jié)劑的(Rspo)家族。目前,該家族由四個結(jié)構(gòu)相關(guān)的分泌配體(Rspo 1-4)組成,所有配體都含有弗林樣結(jié)構(gòu)域和血小板反應(yīng)蛋白結(jié)構(gòu)域。Rspo-1在發(fā)育中的中樞神經(jīng)系統(tǒng)的某些區(qū)域以及腎上腺、卵巢、睪丸、甲狀腺和氣管中表達(dá)。Rspo可以以刺激Wnt/β-catenin信號通路的方式與Frizzled/LRP6受體復(fù)合物相互作用。隨著類器官領(lǐng)域的擴大,R-Spondin 1被廣泛用于類器官細(xì)胞培養(yǎng)中,作為促進(jìn)3D類器官生長和存活的重要成分。

           

          R-性能參數(shù)

           

          蛋白別名(Synonyms)

          R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1

          種屬(Species)

          Mouse

          表達(dá)系統(tǒng)(Source)

          HEK293

          表達(dá)序列(Sequence)

          Ser21-Gln265

          Accession

          Q9Z132

          標(biāo)簽(Tag)

          C-His

          分子量(Molecular Weight)

          The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.

          內(nèi)毒素(Endotoxin)

          < 1 EU/μg by the LAL method.

          純度(Purity)

          > 95% as determined by Bis-Tris PAGE

          活性(Activity)

          ELISA Data: Immobilized Mouse R spondin 1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA.


          Cell Based Assay: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 4 - 20 ng/mL in

          the presence of 5 ng/mL recombinant Human Wnt3a .

          制劑(Formulation)

          Lyophilized from 0.22 μm filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.

          外觀(Appearance)

          凍干粉

           

          儲存條件

           

          -25~-15℃保存,收到貨之后有效期1年。復(fù)溶后, 2 ~8 °C保存,7天有效期。復(fù)溶后, -85~-65℃保存,3個月有效期。

           

          復(fù)溶方法

           

          開蓋之前,先離心。建議復(fù)溶至濃度超過100μg/ml。將凍干的蛋白質(zhì)溶解在 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5)。

           

          注意事項

           

          1.為了您的安全和健康,請穿實驗服并戴一次性手套操作。

          2.本產(chǎn)品僅作科研用途。

           

          Ver. CN20240919

          400-6111-883