產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻
性能參數(shù)
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分子別名（Synonyms） |
IL30;IL-27A;p28;Interleukin-27 |
|
表達區(qū)間及表達系統(tǒng)（Source） |
Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus. It contains Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A).[Accession | Q14213(IL-27B)&Q8NEV9(IL-27A)] |
|
分子量大?。∕olecular Weight） |
The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on SDS-PAGE result. |
|
內(nèi)毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
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純度（Purity） |
> 95% as determined by SDS-PAGE |
|
活性（Activity） |
ELISA Data: Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA . |
|
制劑（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
重構(gòu)方法（Reconstitution） |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
儲存條件
1.The product should be stored at -25~-15℃ for 1 year from date of receipt.
2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
